Table 1. Sensitivity, specificity, time cost and monetary cost of product-sizing analysis, HRM and sequencing analysis.

The estimated times do not include DNA extraction, but include the time to prepare the reaction, the reaction time in the analyzer and the time for the analysis with the corresponding software. The monetary cost is estimated for 27 reactions because with one plate of the HRM, 27 reactions can be tested in triplicate.

	Sensitivity (%)	Specificity (%)	Time cost for 27 reactions (day)	Monetary cost for 1 reaction (euros)	Monetary cost for 27 reactions (euros)
Product sizing analysis	98,2	100	1	0,29	7,83
HRM	96,4	96,3	3	0,63	17,01
Sequencing analysis	89,3	100	4	8,64	233,28