Fig 6. Treatment of Jurkat cells with DHA or DHA/HTF inhibited VEGF mRNA expression.
Jurkat cells (1 × 106/well) were exposed to DHA (0, 10, 20, 40, 80 μM) or DHA/HTF for 48 h, treatment of Jurkat cells with DHA induced a dose-dependent decrease in VEGF expression (a). Compared with DHA treatment, DHA/HTF treatment decreased VEGF expression by 47.77% (p < 0.001). Agarose gel electrophoresis showed that PCR products were of the expected size (b). GAPDH: 258 bp; VEGF: 91 bp. The results represented mean ± standard deviation. ***p<0.001, compared with the control group, # p<0.001, compared with DHA.