FIG 4.

Effect of binding by SsoMCM (A) and/or SsoGINS (C and B) to forked DNA on the distance between the two single-stranded tails of the DNA. SsoMCM and/or SsoGINS were mixed with forked DNA with a 3′ oligo(dT)₂₀ tail labeled at the 3′ end with Cy3 and a 5′ oligo(dT)₂₀ tail labeled at the 5′ end with Cy5 (LD1) (see Table S1 in the supplemental material). Fluorescence intensity was recorded over a range of wavelengths from 545 to 750 nm. Cartoons show the proposed effect of the binding of the protein(s) on the distance between the two single-stranded tails of the forked DNA.