TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | Host strain for plasmids and general use | Lab stock |
| WM3064 | Donor strain for conjugation; ΔdapA | W. W. Metcalf, UIUCa |
| S. oneidensis | ||
| MR-1 | Wild type | ATCC 700550 |
| HG1070 | ΔkatB mutant derived from MR-1 | 19 |
| HG1095 | ΔSO1095 mutant derived from MR-1 | 15 |
| HG1261 | ΔsseA mutant derived from MR-1 | 15 |
| HG1328 | ΔoxyR mutant derived from MR-1 | 19 |
| HG1812 | ΔmdeA mutant derived from MR-1 | 15 |
| HG2364 | ΔccoN mutant derived from MR-1 | 40 |
| HG2903 | ΔcysK mutant derived from MR-1 | 15 |
| HG4056 | ΔmetB mutant derived from MR-1 | 15 |
| ΔcysKC | ΔcysK mutant with copy of cysK integrated into chromosome | 15 |
| Δtriple | ΔmdeA ΔSO1095 ΔsseA mutant derived from MR-1 | This study |
| Δpenta | ΔmdeA ΔSO1095 ΔsseA ΔpsrA ΔsirA mutant derived from MR-1 | This study |
| Plasmids | ||
| pHGM01 | Apr Gmr Cmr suicide vector | 23 |
| pHG101 | Promoterless broad-host-range Kmr vector | 24 |
| pHG102 | pHG101 containing S. oneidensis arcA promoter | 24 |
| pHGEI01 | Integrative lacZ reporter vector | 32 |
| pBBR-Cre | Spr helper plasmid for antibiotic cassette removal | 39 |
| pHGE-PkatB-lacZ | Reporter vector carrying PkatB-lacZ | This study |
| pHGE-Pdps-lacZ | Reporter vector carrying Pdps-lacZ | This study |
| pHGE-PahpC-lacZ | Reporter vector carrying PahpC-lacZ | This study |
| pHGE-PkatG1-lacZ | Reporter vector carrying PkatG1-lacZ | This study |
a
UIUC, University of Illinois at Urbana-Champaign.