FIG 3.
Restoration of v-cyclin reactivation in the absence of p18INK4c did not correspond to differences in neutralizing antibody. Shown is analysis of neutralizing-antibody production in C57BL/6, p18+/−, and p18−/− mice, comparing sera from uninfected (preimmune) or infected (immune; day 42 p.i.) mice, by plaque neutralization assay. WT GHV68 was incubated with or without the indicated dilutions of serum or medium for 1 h, and the percent neutralization was calculated as follows: (PFU in serum-treated samples/PFU in medium-treated controls) × 100. The data represent the means and SEM of the results of two independent experiments, with three independent mice per group.