FIG 4.
Expression analysis of cell cycle-associated genes. Peritoneal cells were collected for RNA isolation and RT-PCR analysis from uninfected C57BL/6 and p18-deficient (p18−/−) mice (A) and C57BL/6 and p18−/− mice infected with either WT GHV68 or vCycKO (KO) (B). Reverse transcription or no-RT control reactions are indicated on the left; the analysis shown represents at least two independent samples tested. Uninfected and infected samples showed no significant difference in cell cycle progression, as indicated by cyclin E levels, in the presence or absence of p18INK4C or in the presence or absence of the viral cyclin.