FIG 7.

HSPA5 is repressed by miR-HSUR5-3p. (A) Ago-bound mRNA fragments, mRNA-Seq reads, and predicted miRNA target sites mapped on the HSPA5 3′ UTR as in Fig. 5. Base-pairing interactions between the WT or Mut target site and miR-HSUR5-3p or miR-BART-13 are shown. Alignment of genomic DNAs of marmoset and human is shown for the miR-HSUR5-3p target sites as in Fig. 5. (B) Luciferase reporter assays were performed with the full-length WT or Mut HSPA5 3′ UTR and miR-HSUR5-3p, miR-BART-13, or a control miRNA. (C and D) Western blot analyses of BiP protein levels in HEK293T or Jurkat cells transfected with miR-HSUR5-3p or a control miRNA. (E) BiP levels 24 h after inhibition of miR-HSUR5-3p by the LNA inhibitor in HVS-infected cells. The bars represent mean values ± SD in at least three experiments. P values were calculated by the Student t test.