FIG 2.

Adhesion of mock-inoculated or EHV-1 (03P37 or 97P70)-inoculated blood CD172a⁺ cells to EC monolayers after preincubation of monoclonal antibodies against α₄β₁ (CD49d/CD29), α_Lβ₂ (CD11a/CD18), and α_Vβ₃ (CD51/CD61) integrins. (A) Flow cytometric analyses of the expression of CD49d, CD29, CD11a/CD18, and CD51/CD61 integrins on CD172a⁺ cells. (B) Cells were mock inoculated or inoculated with EHV-1 strain 03P37 or 97P70 (MOI = 1), preincubated with 10 μg/ml of specific blocking antibodies for 30 min at 4°C, and cocultured with immortalized EC for 2 h at 37°C. As a control, cells were preincubated with isotype-matched antibodies. The number of adherent CD172a⁺ cells per square millimeter was calculated. Experiments were performed three times. The error bars show the SD, and a two-way ANOVA test was performed to evaluate significant differences from controls (*, P < 0.001). (C) Low-magnification confocal images of mock-inoculated or EHV-1 (03P37 or 97P70)-inoculated blood CD172a⁺ cells adherent to EC after preincubation with α_Lβ₂ (CD11a/18) blocking antibody. Nuclei were counterstained with Hoechst (blue). The arrow points to an adhering monocytic cell. All the confocal images shown represent merged z-stacks. Scale bars, 100 μm.