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. 2015 Aug 19;89(21):10912–10923. doi: 10.1128/JVI.01589-15

FIG 5.

FIG 5

Effect of TNF-α on the enhancement of EHV-1 replication in CD172a+ cells upon adhesion to EC. (A) Concentrations of TNF-α in supernatants of CD172a+ cells mock inoculated or inoculated with EHV-1 strain 03P37 or 97P70 (MOI = 1) and cultured on plastic or on top of immortalized EC for 12 h. (B) Expression of EHV-1 gB protein in CD172a+ cells adherent to plastic and cultured in LM supplemented or not with 10 ng/ml of equine recombinant TNF-α (+ TNF-α) for 12 h. (C) Expression of EHV-1 gB protein in CD172a+ cells adherent to EC and cultured for 12 h in LM supplemented or not with 10 ng/ml of TNF-α-neutralizing antibody (+ Anti-TNF-α). The data represent means plus SD of three independent experiments; ns, not significant; *, P < 0.05; **, P < 0.001; ***, P < 0.0001 (by two-way ANOVA test).