FIG 5.

Effect of F3406 on GP2-mediated pH-dependent cell fusion. 293T cells were transfected with pCAGGS-GPC, or empty pCAGGS as a control, and treated 20 h later with compound F3406 (10 μM) or vehicle (DMSO) for 3 h, followed by incubation for 15 min with acidified (pH 5.0) DMEM. After treatment with acidified medium, the cells were washed and placed in DMEM containing 10% FBS. After 5 h, the cells were fixed with 4% PFA-PBS, and syncytium formation visualized by using light microcopy. The panels correspond to representative fields of results observed in three independent experiments.