FIG 2.
trans-Complementation of P in minigenome system. (A) Minigenome activity of P, PNO, and POC. Increasing amounts of P or P deletion mutants were transfected together with other plasmids as described in Materials and Methods. For PNO-POC, the amount of each deletion mutant transfected was one-half of the total amount of P transfected. Renilla luciferase was the reporter gene in the minigenome, and firefly luciferase expression was used as a transfection control. The minigenome activity was measured and normalized as the ratio of Renilla luciferase activity to firefly luciferase activity (relative luciferase activity). (C) Minigenome activity of P, PN, PNO, and POC. Increasing amounts of P or P deletion mutants were used. P values were calculated using Student's t test. Error bars represent the SEMs of data from six replicates. (B and D) Immunoblotting was performed to detect the expression levels of NP, P, and the P deletion mutants. Lanes NC, control.