FIG 6.

Charged residues in the P oligomerization domain are critical for P activity in the minigenome system. (A) Residues found in charged zippers were mutated to alanine and tested in the minigenome system. Increasing amounts of P mutants were transfected with other plasmids as described in Materials and Methods. P values were calculated using Student's t test. Error bars represent the SEMs of data from six replicates. (B) Immunoblotting was performed to detect the expression levels of NP, P, and the P mutants. NC, negative control (no P).