FIG 7.
The C-terminal domain of P binds to L. (A) A schematic representation of the predicted amino acid residues for PIV5 PN, PO, and PC from sequence analysis with MuV P is provided. Mutant names correspond to the domains included in the P deletion mutants. The terminal Flag tag locations as well as additional methionine residues (mmm), used to aid with visualization by radioactive labeling with 35S, are included for each mutant. (B) Interaction between P domains and LdI-III in 293T cells. Cells were transfected with full-length P, P chimeras, or LdI-III or cotransfected with P and LdI-III and labeled with 35S. Cell lysates were precleared with Sepharose G beads and coimmunoprecipitated with monoclonal anti-MuV P (full-length MuV P), anti-PIV5 P (full-length PIV5 P), anti-Flag (chimeric P truncations), or anti-HA (LdI-III) antibody and resolved on a 12.5% SDS-polyacrylamide gel.