FIG 8.

Substrate recombination by wild-type and truncated Int^SSV2 proteins. (A) Integration activity analysis. Wild-type or truncated Int^SSV2 (16 μM) was incubated for 4 h at 65°C with ³²P-labeled A968 (6 nM) and unlabeled P730 (60 nM). The samples were treated with proteinase K and SDS and subjected to electrophoresis in a 3.5% polyacrylamide gel. The gel was exposed to X-ray film. (B) Excision activity analysis. Wild-type or truncated Int^SSV2 (16 μM) was incubated for 4 h at 65°C with ³²P-labeled R943 (6 nM) and unlabeled L755 (60 nM). The samples were processed as described above for panel A. N80 + C81 and N164 + C165 indicate N80 and N164 mixed with C81 and C165, respectively, at a molar ratio of 1. For lane C, Int^SSV2 was omitted. The labeled products are indicated by arrows.