FIG 7.

Spread of Ad14 and Ad14P1 in epithelial xenograft tumors derived from A549 cells. When subcutaneous tumors reached a volume of ∼300 mm³ at day 15 after tumor cell inoculation, 2 × 10⁹ PFU of the Ad14 and Ad14P1 viruses was injected intratumorally. (A) Tumor volumes. Differences in tumor volumes between Ad14 and Ad14P1 are significantly different (P < 0.01) from day 21 on (n = 5). (B) In a second study, established tumors with a volume of ∼300 mm³ were injected intratumorally with 2 × 10⁹ PFU of the Ad14 and Ad14P1 viruses. Tumors were harvested at day 2 and day 7 after Ad injection, homogenized, freeze-thawed, and subjected to titering for infectious viral particles (IU) by limiting dilution assay (n = 3).