TABLE 3.
NA amino acid change (N2 numbering) | Location within NA active siteb | Selection NAIc | Oseltamivir |
Zanamivir |
Peramivir |
Mean infectivity (log10 PFU/ml ± SD) | Mean plaque size ± SD (mm) | Genetic stabilityd (% read) | |||
---|---|---|---|---|---|---|---|---|---|---|---|
Mean IC50 ± SD (nM) | Fold change | Mean IC50 ± SD (nM) | Fold change | Mean IC50 ± SD (nM) | Fold change | ||||||
WT N9 | na | na | 0.40 ± 0.02 | 1.0 | 0.59 ± 0.03 | 1.0 | 0.06 ± 0.03 | 1.0 | 8.1 ± 0.2 | 1.51 ± 0.40 | na |
E119V | Framework | OS | 20.01 ± 1.35 | 49.5 | 1.79 ± 0.13 | 3.0 | 0.08 ± 0.02 | 1.4 | 8.6 ± 0.2 | 1.23 ± 0.16* | V (100) |
P120S | – | ZA | 0.40 ± 0.01 | 1.0 | 0.73 ± 0.03 | 1.2 | 0.06 ± 0.04 | 1.0 | 8.5 ± 0.2 | 1.35 ± 0.36 | ND |
Q136K | – | ZA | 0.20 ± 0.04 | 0.5 | 71.48 ± 4.47 | 121.1 | 3.23 ± 0.89 | 53.9 | 7.6 ± 0.2 | 0.74 ± 0.21* | K (100) |
G147R | – | ZA | 0.26 ± 0.02 | 0.6 | 1.03 ± 0.07 | 1.7 | 0.08 ± 0.04 | 1.5 | 8.3 ± 0.5 | 1.28 ± 0.23* | ND |
I222M | Framework | OS | 7.32 ± 0.27 | 18.3 | 2.37 ± 0.74 | 4.0 | 0.24 ± 0.02 | 4.0 | 8.1 ± 0.3 | 1.05 ± 0.22* | M (100) |
A246T | – | ZA | 2.47 ± 0.72 | 6.2 | 15.01 ± 1.93 | 25.4 | 0.43 ± 0.07 | 7.2 | 7.5 ± 0.1 | 1.51 ± 0.31 | T (100) |
E259D | – | OS | 0.41 ± 0.02 | 1.0 | 0.75 ± 0.09 | 1.3 | 0.13 ± 0.02 | 2.2 | 8.0 ± 0.0 | 1.54 ± 0.26 | ND |
H274Y | Framework | Inducede | 36.13 ± 12.53 | 90.3 | 1.41 ± 0.18 | 2.4 | 0.67 ± 0.19 | 11.2 | 7.7 ± 0.1 | 0.89 ± 0.22* | Y (100) |
R292K | Catalytic | OS | 27,832.00 ± 31,020.67 | 68,891.1 | 30.27 ± 5.49 | 51.3 | 116.43 ± 6.65 | 2,067.9 | 8.5 ± 0.1 | 1.00 ± 0.16* | K (100) |
D339G | – | ZA | 0.38 ± 0.02 | 0.9 | 0.63 ± 0.10 | 1.1 | 0.10 ± 0.01 | 1.7 | 8.4 ± 0.2 | 1.62 ± 0.44 | ND |
See Table 1, footnote a.
See Table 1, footnote b.
See Table 1, footnote c.
See Table 1, footnote d.
See Table 1, footnote e.