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. 2015 Oct 20;9:5727–5736. doi: 10.2147/DDDT.S92355

Figure 3.

Figure 3

The effect of 4-HIL on the TACE/TIMP3 system in IR 3T3-L1 adipocytes.

Notes: Cells were incubated with the indicated concentrations of 4-HIL or 10 µM PIO for 24 hours, and then total proteins were extracted and analyzed by immunoblotting for TACE and TIMP3. The same blot was then stripped and reprobed with anti-β-actin antibody to ensure equal protein loading (A). PIO was used as a positive control. The figure shows a representative gel from one experiment. The values are presented as the mean ± SE of three independent experiments. **P<0.01, ***P<0.001 vs the IR-like group (0 µM 4-HIL) for TACE protein expression; #P<0.05, ##P<0.01, ###P<0.001 vs the IR-like group (0 µM 4-HIL) for TIMP3 protein expression (B).

Abbreviations: 4-HIL, (2S,3R,4S)-4-hydroxyisoleucine; TACE, TNF-α converting enzyme; TIMP3, Tissue inhibitor of metalloproteinase 3; IR, insulin resistance; PIO, pioglitazone; SE, standard error.