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. 2015 Oct 19;8:2989–3001. doi: 10.2147/OTT.S91511

Figure 5.

Figure 5

Luteolin inhibits IL-6-induced MMP secretion of PANC-1 and SW1990 cell lines.

Notes: Two cell lines were treated with IL-6 at 100 ng/mL for 72 hours in FBS-free media and in the last 24 hours luteolin was added to reach a concentration of 80 μM. (A) Western blotting assay was performed to determine protein level of MMP2, MMP7, and MMP9; GAPDH was used as an internal control. (B) Fold change in mRNA expression of MMP2, MMP7, and MMP9 in SW1990 cells. (C) Fold change in mRNA expression of MMP2, MMP7, and MMP9 in PANC-1 cells. Data represent mean ± SEM, n=3, *P<0.05.

Abbreviations: MMP, metalloproteinase; mRNA, messenger RNA; SEM, standard error of mean; STAT3, signal transducer and activator of transcription 3; IL-6, interleukin-6.