Table 2.
Cell wall-associated proteins identified in C. neoformans cap67 cell wall preparations. The proteins areorganized according to GO functions. Minor and unknown functions are omitted, but can be seen in a complete Supplemental Table 1.
| Codenumber* | Proteinname | CW** | EV** |
|---|---|---|---|
| Transport | |||
| CNAG_02817 | GTP-binding protein ypt2 | - | CnHcPbSc |
| CNAG_02974 | voltage-dependent anion channel protein 2 | Af | Cn |
| CNAG_04851 | transitionalendoplasmicreticulumATPase | Ca Pb | HcSc |
| CNAG_04904 | clathrin heavy chain | - | - |
| CNAG_06101 | ADP, ATP carrier protein | Ca | CnHcPbSc |
| CNAG_06377 | solute carrier family 25, member 3 | - | Cn |
| CNAG_00499 | solute carrier family, member 20/29 | - | Hc |
| CNAG_06096 | tricarboxylatecarrier | - | - |
| Response to stress | |||
| CNAG_00334 | hsp75-like protein | Af Ca Sc | CnHcPbSc |
| CNAG_01727 | hsp71-like protein | Af Ca Sc | CnHcPbSc |
| CNAG_01750 | hsp72-like protein | Ca Sc | CnHcPbSc |
| CNAG_03891 | hsp60-like protein | Ca Pb | CnHcPbSc |
| CNAG_05199 | chaperoneDnaK | Af Ca | HcPbSc |
| CNAG_06150 | hsp90-like protein | Af Ca PbSc | CnHcPbSc |
| CNAG_06208 | heat shock 70kDa protein 4 | Af Ca PbSc | HcPbSc |
| CNAG_06443 | glucose-regulatedprotein | Ca Sc | CnHcPbSc |
| ATP biosyntheticprocess | |||
| CNAG_01004 | ATP synthase F1, delta subunit | - | CnHcPb |
| CNAG_04439 | V-type proton ATPase subunit B | - | CnSc |
| CNAG_05750 | ATP synthase subunit alpha, mitochondrial | Ca Pb | CnHcPbSc |
| CNAG_05918 | ATP synthasesubunit beta, mitochondrial | Ca Pb | CnHcPbSc |
| CNAG_06400 | proton-efflux P-type ATPase | Ca Sc | CnHcPbSc |
| CNAG_02326 | V-type proton ATPase catalytic subunit A | - | HcSc |
| Translation | |||
| CNAG_00034 | largesubunitribosomalprotein L9e | - | CnPbSc |
| CNAG_00096 | small subunit ribosomal protein S3 | - | HcPbSc |
| CNAG_00640 | small subunit ribosomal protein S4-A | Ca | CnHcSc |
| CNAG_00656 | largesubunitribosomalprotein L7e | Ca Sc | CnPbSc |
| CNAG_00672 | small subunit ribosomal protein S9 | Sc | CnHc |
| CNAG_01628 | small subunit ribosomal protein S20 | Ca Sc | CnHc |
| CNAG_01024 | large subunit ribosomal protein L18-A | - | Cn |
| CNAG_02928 | largesubunitribosomalprotein L5e | Ca Sc | CnHcPbSc |
| CNAG_00779 | largesubunitribosomalprotein L27e | Sc | CnHc |
| CNAG_00952 | smallsubunitribosomalprotein S6e | Ca | HcSc |
| CNAG_00953 | smallsubunitribosomalprotein S13e | Ca | CnPbSc |
| CNAG_01884 | large subunit ribosomal protein L3 | Ca Sc | CnHcPb |
| CNAG_01486 | large subunit ribosomal protein L15-A | Ca PbSc | CnSc |
| CNAG_01951 | small subunit ribosomal protein S22-A | - | CnHcSc |
| CNAG_02144 | large subunit ribosomal protein L1-A | - | CnHcPb |
| CNAG_02234 | largesubunitribosomalprotein L6e | Ca Pb | Cn |
| CNAG_02331 | small subunit ribosomal protein S9 | Ca | Cn |
| CNAG_03000 | smallsubunitribosomalprotein S19e | Ca | Cn |
| CNAG_03053 | large subunit ribosomal protein L23 | - | CnSc |
| CNAG_03198 | smallsubunitribosomalprotein S8e | Ca | CnSc |
| CNAG_03510 | largesubunitribosomalprotein L36e | - | HcPb |
| CNAG_03577 | large subunit ribosomal protein LP0 | AfPbSc | HcSc |
| CNAG_03739 | large subunit ribosomal protein L10-like | Ca Sc | CnHcSc |
| CNAG_03780 | small subunit ribosomal protein S16 | Ca | CnSc |
| CNAG_04004 | small subunit ribosomal protein S1 | Ca Sc | CnHcPb |
| CNAG_00409 | large subunit ribosomal protein L37a | - | Cn |
| CNAG_04021 | large subunit ribosomal protein L24 | - | Cn |
| CNAG_00494 | small subunit ribosomal protein S0 | Af Ca Sc | CnSc |
| CNAG_04445 | smallsubunitribosomalprotein S7e | Ca Sc | CnHcPbSc |
| CNAG_04726 | large subunit ribosomal protein L18Ae | Ca | Cn |
| CNAG_04762 | largesubunitribosomalprotein L4e | Ca | CnHcPbSc |
| CNAG_04799 | largesubunitribosomalprotein L14e | - | CnPbSc |
| CNAG_04883 | small subunit ribosomal protein S18 | - | CnHcPb |
| CNAG_00417 | elongationfactor 1-gamma | Pb | CnPbSc |
| CNAG_00785 | ATP-dependent RNA helicase eIF4A | Af Ca Sc | CnHcPbSc |
| CNAG_04609 | argonaute | - | CnHc |
| CNAG_05555 | large subunit ribosomal protein L7Ae | PbSc | CnSc |
| CNAG_06105 | elongationfactor 1-alpha | Ca PbSc | CnHcPbSc |
| CNAG_06605 | small subunit ribosomal protein S2 | AfSc | CnHcPbSc |
| CNAG_00689 | largesubunitribosomalprotein L22e | Pb | Pb |
| CNAG_05232 | large subunit ribosomal protein L8 | Ca PbSc | CnHcSc |
| CNAG_06231 | large subunit ribosomal protein L13 | - | - |
| CNAG_06447 | large subunit ribosomal protein L22 | Ca Sc | CnSc |
| Proteinmetabolism | |||
| CNAG_04009 | aminopeptidase 2 | - | HcPbSc |
| CNAG_00150 | peptidase | - | Cn |
| CNAG_00919 | carboxypeptidase D | Pb | CnSc |
| CNAG_01890 | 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase | Ca PbSc | CnHcPbSc |
| CNAG_02500 | calnexin | Pb | Cn |
| CNAG_02966 | carboxypeptidase | - | Sc |
| CNAG_03507 | mitochondrial peptidase subunit beta | - | CnHc |
| CNAG_04604 | tryptophan-tRNAligase | - | - |
| CNAG_04625 | cerevisin | Ca Pb | CnHcPbSc |
| CNAG_05725 | ketol-acidreductoisomerase, mitochondrial | Ca | CnHcSc |
| CNAG_05932 | peptidyl-prolylcis-transisomerase D | Pb | HcPb |
| CNAG_06026 | aspartateaminotransferase | Pb | HcPbSc |
| CNAG_06088 | aspartateaminotransferase, mitchondrial | - | - |
| CNAG_00136 | ubiquitin-activatingenzyme E1 | - | Sc |
| CNAG_05722 | alanine-tRNAligase | Sc | Sc |
| CNAG_04906 | 26S protease regulatory subunit 10B | - | - |
| CNAG_05886 | ubiquitin-conjugatingenzyme E2 | Pb | - |
| CNAG_06755 | threonine-tRNAligase | - | Sc |
| Carbohydratemetabolism | |||
| CNAG_00061 | citratesynthase, mitochondrial | Ca | CnHcPbSc |
| CNAG_00937 | aconitatehydratase, mitochondrial | Ca Pb | HcPbSc |
| CNAG_00964 | glutamine-fructose-6-P transaminase | - | - |
| CNAG_01820 | pyruvatekinase | Ca Sc | CnHcSc |
| CNAG_01896 | alcoholdehydrogenase | Ca Pb | CnPbSc |
| CNAG_01984 | transaldolase | Ca Sc | CnHcPbSc |
| CNAG_02035 | triose-phosphateisomerase | Ca Sc | HcPbSc |
| CNAG_02818 | glycine cleavage system T protein | - | CnHc |
| CNAG_03072 | enolase | Af Ca PbSc | CnPbSc |
| CNAG_03225 | malatedehydrogenase, NAD-dependent | Ca | CnHcPbSc |
| CNAG_03358 | phosphoglyceratekinase | Af Ca Sc | CnHcPbSc |
| CNAG_03525 | alpha, alpha-trehalase | - | - |
| CNAG_03674 | oxoglutaratedehydrogenase, E1 | - | Hc |
| CNAG_03725 | dTDP-4-dehydrorhamnose reductase | Pb | Cn |
| CNAG_04640 | ATP-citrate synthase subunit 1 | - | CnHcSc |
| CNAG_04659 | pyruvatedecarboxylase | Ca PbSc | CnSc |
| CNAG_04969 | UDP-glucose 6-dehydrogenase | - | Cn |
| CNAG_05653 | malatesynthase A | - | CnHcSc |
| CNAG_05907 | pyruvatecarboxylase | - | CnHcSc |
| CNAG_06313 | phosphoglucomutase | Ca Pb | HcPbSc |
| CNAG_06666 | starchphosphorylase | - | Cn |
| CNAG_06699 | glyceraldehyde-3-phosphate dehydrogenase | Af Ca PbSc | CnHcPbSc |
| CNAG_06770 | fructose-bisphosphatealdolase 1 | Ca PbSc | HcPbSc |
| CNAG_05351 | phosphomannomutase | Sc | HcSc |
| CNAG_02748 | UTP-glucose-1-Puridylyltransferase | Ca | CnSc |
| CNAG_04217 | phosphoenolpyruvatecarboxykinase | Pb | HcSc |
| CNAG_06501 | 1,3-beta-glucanosyltransferase | Ca PbSc | CnHcSc |
| Lipidmetabolism | |||
| CNAG_02099 | fatty acid synthase subunit beta, fungi type | - | HcSc |
| CNAG_02100 | fatty acid synthase subunit alpha, fungi type | Sc | CnSc |
| CNAG_02562 | acyl-CoAdehydrogenase | - | - |
| CNAG_04308 | 3-hydroxyacyl-CoA dehydrogenase | - | Hc |
| CNAG_04869 | carboxylesterase | - | Cn |
| CNAG_07004 | dihydrolipoyldehydrogenase | - | CnPbSc |
| Nucleicacidmetabolism | |||
| CNAG_01395 | ribose-5-phosphate isomerase | Ca | Pb |
| CNAG_00165 | methylthioadenosinephosphorylase | Pb | Hc |
| CNAG_02285 | nucleosidediphosphatekinase | - | CnHcPbSc |
| CNAG_04577 | nucleoside-diphosphatekinase | - | CnHcPbSc |
| Oxidation/Reduction | |||
| CNAG_00452 | isovaleryl-CoAdehydrogenase | - | Hc |
| CNAG_00938 | cytochrome c peroxidase, mitochondrial | Af | Hc |
| CNAG_01492 | hypotheticalprotein | - | - |
| CNAG_01594 | glycinedehydrogenase | - | Hc |
| CNAG_01981 | sulfide:quinoneoxidoreductase | - | - |
| CNAG_02399 | glutathione-disulfidereductase | PbSc | Cn |
| CNAG_02801 | thioredoxin | Ca Pb | CnSc |
| CNAG_03482 | peroxiredoxin | Ca Sc | CnSc |
| CNAG_03618 | NADPH2:quinone reductase | Ca Sc | HcSc |
| CNAG_04981 | catalase | Pb | CnHcPbSc |
| CNAG_06628 | aldehydedehydrogenase | PbSc | CnHcSc |
| CNAG_06917 | thiol-specific antioxidant protein 3 | Ca Pb | CnHcPb |
| CNAG_00162 | alternative oxidase, mitochondrial | - | - |
| CNAG_03936 | NAD(P)H:quinoneoxidoreductase, type IV | - | PbSc |
| CNAG_05753 | N-acetyl-gamma-glutamyl-Preductase | - | - |
| CNAG_05721 | multifunctional beta-oxidation protein | - | Hc |
| CNAG_05069 | ubiquinol-cytochrome c reductase sub. 10 | - | - |
| CNAG_06431 | acyl-CoA oxidase | - | - |
| Signaling | |||
| CNAG_01523 | CMGC/MAPK/P38 protein kinase | - | Sc |
| CNAG_05235 | protein BMH2 | Ca PbSc | CnHcPbSc |
| CNAG_05465 | guanine nucleotide-binding protein | Af Ca PbSc | HcSc |
| Nucleosomeassembly | |||
| CNAG_06745 | histone H3 | - | - |
| CNAG_06746 | histone H2B | Ca Pb | CnHcPbSc |
| Cytoskeleton | |||
| CNAG_00483 | actin | Ca PbSc | CnHcPbSc |
| CNAG_01840 | tubulin beta chain | - | CnPbSc |
| CNAG_03787 | tubulin alpha-1A chain | Ca | Cn |
| CNAG_04948 | tubulin beta | - | - |
*
Proteins were classified by the Fungal Secretome Database (FSD) into NS (nonclassical secretion, predicted by SecretomeP 1.0f) (dark gray code number), SP or SP3 (containing signal peptide identified by SignaIP 3.0, SigPred, SigCleave or RPSP) (light gray code number) and not classified as secretory (white code number).
**
Orthologs previously reported in cell wall (CW) or extracellular vesicles (EV) from H. capsulatum (Hc), P. brasiliensis (Pb), S. cerevisiae (Sc), C. albicans (Ca), and A. fumigatus (Af) are indicated.