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. 2015 Oct 27;9:136. doi: 10.3389/fnana.2015.00136

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Copyright © 2015 Miquelajáuregui, Sandoval-Schaefer, Martínez-Armenta, Pérez-Martínez, Cárabez, Zhao, Heide, Alvarez-Bolado and Varela-Echavarría.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lxh2 and Lhx9 misexpression in the Lhx5^-/- posterior hypothalamus. Whole-mount ISH for Lhx2 (A,B) and Lhx9 (C,D) in control and Lhx5 mutant E12.5 embryos (anterior is to the left), showing ectopic expression of both markers in the posterior hypothalamus of Lhx5 mutants (asterisks in B,D). (A,B) The Lhx2-expressing region in the AH was present in both conditions, but the tuberal expression domain appeared shortened along the anterior–posterior axis (brackets in A,B). (C,D) Lhx9 is expressed at low levels in the anterior hypothalamus in both controls and Lhx5 mutants, in a region that might correspond to the IDv domain (empty arrowheads). Note that expression of both genes in the Th seems unaltered in Lhx5 mutants. Abbreviations: AH, anterior hypothalamus; Th, thalamus; IDv, ventral intrahypothalamic diagonal; Mb, midbrain; PT, pretectum; PTh, prethalamus; Tu, tuberal. Scale bar: 200 μm.