Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The cph2 upc2 double mutant is completely defective in hypoxic growth. WT (HLY4151), cph2 (HLY4152), cph2+CPH2 (HLY4195), upc2 (54), and two independently obtained cph2 upc2 double mutant strains (HLY4007 and HLY4008) were grown in YPD to an OD600 of ∼1. Cells were diluted to an OD600 of 0.1 and then serially diluted 5-fold, spotted (1.25 μl) onto YPD and SC plates, and grown at 37°C in normoxia or 0.2% O2 and 5% CO2 for a day before pictures were taken.
