FIG 2.

Degeneracy of transposable elements and ORF10 located near the solanapyrone gene cluster. (A) Sliding-window analyses of the nucleotide composition were conducted for the full-length functional Molly transposon from Stagonospora nodorum (1,866 bp; GenBank accession number AJ488502) and pseudogenes (P4 to P6) found in the subtelomeric region (P4 and P5, 1,866 bp; P6, 1,867 bp), including the 5′ and 3′ TA insertion sites, terminal inverted repeats, and putative transposase. Values for GC content (in percent; black line), RIP index I (number of ApT residues/number of TpA residues; blue line), and RIP index II [(number of CpA residues + number of TpG residues)/(number of ApC residues + number of GpT residues; red line] are shown. These values were calculated across a 200-bp window, which was slid in 20-bp increments across the full-length elements. Horizontal solid lines, the 50% GC mark; dashed lines, a RIP index value of 1. (B) Sliding-window analyses of the nucleotide composition of ORF10 plus its 5′ and 3′ flanking regions (2,029 bp in total) (top) and the homologous P450 gene (2,020 bp, GenBank accession number XP_007784573) (bottom). The black box below each graph indicates the position of the coding regions of the sequences. ORF10 with a 5′ flanking region (5′F) and a 3′ flanking region (3′F) and the corresponding homologous region in the P450 gene are shaded for comparison.