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. Author manuscript; available in PMC: 2015 Oct 27.
Published in final edited form as: J Immunol. 2015 Jan 12;194(4):1591–1601. doi: 10.4049/jimmunol.1402214

Figure 3. KIR3DL2 dimerisation is not necessary for binding to B27 FHC dimers.

Figure 3

A Left hand panel. IL-2 production by wild type (WT) and KIR3DL2CD3□C281AC315A (CYS-) mutant reporter cells in response to stimulation with 221B27 cells. Representative data from 1 of 3 independent experiments. B Representative FACS stain of WT and C281AC315A KIR3DL2CD3□reporter cells with the indicated KIR3DL2 antibodies (DX31 and D1A). C. Left hand panels. Western blots of cell surface immunopreciptates from KIR3DL2CD3□ reporter cells with the D1A antibody resolved by non-reducing (-DTT) or reducing (+DTT) SDS PAGE probed with HRP-conjugated anti CD3εRight hand panels. Western blots of cell surface immunopreciptates from jurkat cells expressing HA-tagged KIR3DL2 with the D1A antibody resolved by non-reducing (-DTT) or reducing (+DTT) SDS PAGE probed with HRP-conjugated anti HA.