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. 2015 Oct 27;5:15488. doi: 10.1038/srep15488

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Relative abundance of genera was firstly transformed into Z-scores as described in Materials and Methods. Then, synchronized genera defined as genera that have similar temporal variations were identified by K-means clustering. (A) Relative abundances of “center” genera of resulted clusters at different sampling months. (B) The ratio of within-cluster SS and between-cluster SS as function of the number of clusters. This curve helps to determine the appropriate parameter K in K-means clustering. (C) Total abundance of each cluster and corresponding representation phylum. Phylum assignments of most abundant genera that take up >50% of cluster abundances were used to represent each cluster.