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. 2015 Oct 27;5:242. doi: 10.3389/fonc.2015.00242

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Copyright © 2015 Reuter, Staege, Kühnöl and Föll.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stability of EFT makers in OX40L transgenic EFT cells. Expression of the indicated markers was assessed by RT-PCR in cells without transfection (1), after transfection with empty pIRES-eGFP vector (2), or after transfection with OX40L in vector pIRES-eGFP (3). Actin beta (ACTB) served as housekeeping control. The neomycin-resistance cassette (NeoR) and enhanced green fluorescent protein (eGFP) served as markers for the presence of the vector in the cells. Transfected and wild-type Ewing sarcoma cells expressed the Ewing sarcoma-specific EWS-FLI1 oncofusion transcripts as well as the Ewing sarcoma-associated factors lipase I (LIPI), lipase H (LIPH), CD99, cyclin D1 (CCND1), janus kinase 1 (JAK1), and cytochrome P450 family member 26B1 (CYP26B1).