Figure 7.

SO_3166-SO_3165 represses motility in S. oneidensis. (A) In-frame deletion mutant of the toxin gene SO_3166 and the SO_3165-SO_3166 operon. Primer LF indicates the long forward primer and SF indicates the short forward primer. Primers SR and LR indicate the short reverse and long reverse primers, respectively. PCR products are indicated with the expected sizes using genomic DNA from the wild-type (WT) and the deletion mutants. (B) Swimming motility test for the SO_3165-3166 deletion mutant versus WT (left plate), and for wild-type MR-1 co-expressing SO_3165 and SO_3166 versus empty vector (right plate). Two independent cultures were evaluated for each; only one representative image is shown here.