Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strains/Plasmids | Descriptiona | Source |
|---|---|---|
| E. coli strains | ||
| BL21(DE3) | F-ompT hsdSB(rB-mB-) gal dcm λ(DE3) Ω PtacUV5::T7 polymerase | Novagen |
| K12 BW25113 | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | (Baba et al., 2006) |
| WM3064 | thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360) Δ(araBAD)567 ΔdapA1341::[erm pir(wt)] | W. Metcalf, UIUC |
| DH5α | F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen |
| S. oneidensis strains | ||
| MR-1 | Wild type | (Shi et al., 2013) |
| ΔSO_3165–3166 | In frame deletion of SO_3165–3166 operon | This study |
| ΔSO_3166 | In frame deletion of SO_3166 gene | This study |
| Plasmids | ||
| pCA24N | CmR; lacIq, IPTG inducible expression vector in E. coli | (Kitagawa et al., 2005) |
| pCA24N-SO_3165 | CmR; lacIq, PT5-lac:: SO_3165 | This study |
| pCA24N-SO_3166 | CmR; lacIq, PT5-lac:: SO_3166 | This study |
| pCA24N-SO_3165–3166 | CmR; lacIq, PT5-lac:: SO_3165–3166, IPTG inducible expression vector in E. coli | This study |
| pHGE | pHGE-Ptac, KmR, IPTG inducible expression vector in S. oneidensis | (Shi et al., 2013) |
| pHGE-SO_3165 | KmR; expression vector for SO_3165 | This study |
| pHGE-SO_3166 | KmR; expression vector for SO_3165 | This study |
| pHGE-SO_3165–3166 | KmR; expression vector for SO_3165–3166 | This study |
| pHGM01 | GmR; CmR; ApR; sacB; Ori-R6K; suicide plasmid for generating in-frame deletions | (Shi et al., 2013) |
| pHGM01-SO_3166 | pHGM01 containing the PCR fragments for deleting SO_3166 | This study |
| pHGM01-SO_3165–3166 | pHGM01 containing the PCR fragments for deleting SO_3165–3166 | This study |
| pET28b | ||
| pET28b-SO_3165–3166-CHis | KmR, lacIq, pET28b PT7-lac:: SO_3165–3166 with C-terminal His-tagged | This study |
| pET28b-SO_3165 | KmR, lacIq, pET28b PT7-lac:: SO_3165 | This study |
| pET28b-SO_3165-cHis | KmR, lacIq, pET28b PT7-lac:: SO_3165 with His-tagged at C-terminal | This study |
| pBS(Kan) | KmR; pBS(Kan) | (Canada et al., 2002) |
| pBS(Kan)-PSO_3165–3166 | KmR; pBS(Kan) with 500-nt upstream and coding region of SO_3165–3166 | This study |
| pBBR-Cre | Helper vector for removing antibiotic cassette | (Fu et al., 2014) |
| pHGEI01 | Integrative lacZ reporter vector | (Fu et al., 2014) |
| pHGEI01-PSO_3165–3166 | Integrative lacZ reporter vector carrying 300-nt upstream of SO_3165–3166 | This study |
a. CmR, KmR, GmR and ApR indicate chloramphenicol, kanamycin, gentamycin and ampicillin resistance, respectively.