Figure 2.

Effect of Sorbitol on the accumulation of glycerol and resistance to high salt stress. (A) Animals grown in medium containing 10X peptone or 5% sorbitol induce expression of the glycerol-3-phosophate dehydrogenase reporter pgdh-1::GFP, as determined by immunoblot analysis for GFP. (B) Whole animal glycerol levels were elevated in wild-type (N2) worms were grown on 5% sorbitol or 4X NaCl relative to controls (^***p < 0.05, t-test). (C) Either 5% sorbitol or 4X NaCl is sufficient to confer resistance to higher levels of NaCl through osmotic pre-adaptation (^***p < 0.05, t-test).