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. 2015 Oct 27;5:14919. doi: 10.1038/srep14919

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Copyright © 2015, Macmillan Publishers Limited

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Flag-tagged dominant negative mutants of EFA6s, EFA6A (E621K), EFA6B (E651K) or EFA6D (E604K), were coexpressed with Arf6-Flag and with or without HA-Dyn2 in HEK293T cells. After 24 hr of expression, the levels of GTP-Arf6 were detected as in Fig. 1A (upper panels). Lower panel represents the mean ± SEM of the levels of GTP-Arf6 from five independent experiments. Statistical significance was calculated using Tukey multiple comparison test; **P < 0.01 and N.S., not significant.