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. 2015 Oct 27;5:14919. doi: 10.1038/srep14919

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HA-Dyn2 and Flag-tagged deletion mutants of EFA6B were coexpressed in HEK293T cells. After immunoprecipitation of Flag-EFA6B mutants, coimmunoprecipitated Dyn2 was assessed by western blotting probed with anti HA-antibody.