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. 2015 Oct 27;5:15465. doi: 10.1038/srep15465

Figure 4. Increased BDNF in fat-1 transgenic mice enhanced Purkinje cell survival after STZ-treated animal model.

Figure 4

(A–C) BDNF and its receptor, TrkB protein levels were analyzed by immunoblotting at day 18 after the fifth STZ injection into wild-type (n = 7/group) and fat-1 transgenic mice (n = 9/group). The ratio of BDNF and TrkB vs. actin were shown in the right panel (one-way ANOVA with post hoc Newman-Keuls test, ***P < 0.001, vs. control. Data present mean ± S.D. of three independent experiments). (D) Representative confocal images showed the BDNF immunoreactivity was found in Purkinje cells of STZ-induced fat-1 transgenic mice, but TrkB was hardly detected. Scale bar = 10 μm. (F–H). BDNF and TrkB receptor-associated downstream signaling molecules, Akt and CREB protein levels were analyzed by immunoblotting in STZ-induced wild-type (n = 7/group) and fat-1 transgenic mice (n = 9/group). The ratio of phospho-Akt and phospho-CREB vs. actin were shown in the right panel (one-way ANOVA with post hoc Newman-Keuls test, **P < 0.01, vs. control. Data present mean ± S.D. of three independent experiments).