Figure 2.

PA attenuated b-catenin signaling in cardiomyocytes. (A) Subcellular localization of active b-catenin in cardiomyocytes incubated with BSA (Cont) or PA (400 μM) was examined by indirect immunofluorescence (red, b-catenin and blue, DAPI; scale bar, 20 μm). (B) Levels of cytosolic and nuclear b-catenin were determined by western blot in subcellular fractions. GAPDH or histone-3 was used as internal control respectively. (C) Protein expression of survivin and BCL2 was examined by western blot. Intensities were quantified and normalized against the level of GAPDH or histone-3 and expressed as percentage of protein abundance at control (B and C). Data are means±s.e.m. of three independent experiments. **P<0.01 vs Cont. Cont, control; PA, palmitate. A full colour version of this figure is available at http://dx.doi.org/10.1530/JME-15-0155.