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Dual-fluorophore labeling of hsImportin-β. (A) Cartoon model of hsImportin-β showing the positions of AzF incorporation and their approximate distances to the sfGFP fluorophore. The figure was created using Pymol v0.99 and PDB entries 1QGK and 1KYS. (B) Importin proteins with or without genetically fused sfGFP-tag were purified from E. coli and labeled with DBCO-Fl.-545 where indicated. Fluorescence was analyzed using a Typhoon phosphoimager. To see this figure in color, go online.
