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. 2015 Nov;146(5):343–356. doi: 10.1085/jgp.201511456

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© 2015 Cherny et al.

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Figure 9. — The W176F mutation in the dinoflagellate kH_V1 accelerates channel opening. Families of currents in WT kHv1 (A and B) and in the W176F mutant (C and D), studied at pH_o 7 (A and C) or 6 (B and D), as indicated, all at pH_i 7. Note the different time scales. Voltage steps were applied in 10-mV increments to the final voltage indicated. The holding potential was −60 (A and C) or −40 mV (B and D). (E) Replacement of Trp¹⁷⁶ in the dinoflagellate kH_V1 with Ala, Ser, or Phe hastens channel opening to the same extent. The activation time constant, τ_act, was determined by fitting the turn-on of current with a single rising exponential. The WT channel kinetics (mean ± SEM; n = 5–14) was extracted from data for a previous study, all at symmetrical pH 7.0 (Smith et al., 2011). Single mutants, as indicated, were expressed in HEK-293 or COS-7 cells. Measurements were performed in whole-cell configuration at symmetrical pH 7. Data from each cell are connected by lines.