Figure 4.

Doughnut diagrams integrating IGF2 gene expression data with somatic copy number changes and DNA methylation alterations of the IGF2/H19 locus. CAs and ACBTs were mainly characterized by normal IGF2 expression, absence of copy number changes, and normal DNA methylation levels of the H19 ICR. In contrast all PCCs showed overexpression of IGF2, where all samples with somatic copy number changes showed hypermethylation of the H19 ICR. Seventeen out of 20 ACCs presented with overexpression of IGF2 and this mainly correlated with somatic copy number changes of chr11p15.5 and hypermethylation of the H19 ICR. Somatic copy number changes of chr11p15.5 were not exclusively associated with high IGF2 expression levels as a ACC sample (57) with an UPD-like genotype had IGF2 expression levels comparable to the samples from patients with Conn's adenoma.