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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Immunol. 2015 Aug 3;195(5):2122–2133. doi: 10.4049/jimmunol.1500396

FIGURE 6.

FIGURE 6

Calcineurin-NFAT signaling regulates PAR2 and CRAC channel mediated induction of inflammatory mediators from AECs. (A) Summary of the effect of calcineurin inhibitor CsA (500 nM) on IL-6, IL-8, PGE2 and GM-CSF induction in response to the PAR2 agonistic peptide SLIGRL (100 µM) in NHBE cells. Cells were stimulated for 2 hours for PGE2 induction, 8 hours for IL-6 and IL-8 induction and 24 hours for GM-CSF induction. Data is shown as fold change in cytokine production (actual concentrations: IL-6: 18–70 pg/mL; IL-8: 55–200 pg/mL; PGE2: 2300–4500 pg/mL; GM-CSF: 75–220 pg/mL). (B) Summary of the change in cytokine levels in NHBE cells (12 hour stimulation) evoked by CRAC channel activation. NHBE cells were treated with TG (250 nM) and PDBu (50 nM) in the absence and presence of CsA (250 nM). N=3 replicates, 3 independent experiments.