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Transient absorption changes of wild-type ChR2 at 1717 cm−1 in H2O (black) and at 1708 cm−1 in D2O (red). These frequencies correspond to the C=O stretching vibration of E90 in water and in deuterium oxide, respectively. Absorbance changes from 300 ms to 90 s were recorded by time-resolved rapid-scan FTIR spectroscopy and merged to the time-resolved QCL data. To see this figure in color, go online.
