Figure 6.
Deleterious effects of actinonin on mitochondrial protein synthesis do not arise from inhibiting Pdf. (A) Immunoblot of whole cell lysates of Pdf knockdown with independent siRNAs compared with scrambled control (sc) or without siRNA (−) in MEFs ectopically expressing an HA-tagged Pdf. (B) Immunoblotting of Pdf knockdown MEFs treated with or without actinonin for 6 h. (C) Extended 35S metabolic labeling for 3 h in MEFs with Pdf knockdown treated with and without actinonin. The arrow indicates accumulation of aberrant translation product.