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. 2015 Oct 26;211(2):455–468. doi: 10.1083/jcb.201506114

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© 2015 Domingo Sananes et al.

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Figure 2. — Trypanosome differentiation is an irreversible bistable switch. (A) Time course of EP procyclin expression after trypanosome exposure to 6 mM CA. Procyclin expression was detectable within 1 h and maximum expression was achieved at 3 h. Some reduction in EP procyclin expression was routinely observed at 24 h after induction in all experiments. Quantitation of the EP procyclin expression at each time point is shown. (B) Signal sensitivity of differentiation. Parasites were exposed to a titration of CA at 27°C and monitored for the expression of EP procyclin at 24 h. Quantitation of the EP procyclin expression at 24 h is shown. (C) Commitment to differentiation. Parasites were exposed to 6 mM CA for 1, 2, or 3 h and then washed free of CA. The expression of EP procyclin was then monitored at 24 h. Cells maintained in CA throughout the 24 h of the experiment were also assayed. Quantitation of the EP procyclin expression at each time point is shown. (D) Differentiation is irreversible. Parasites were exposed to 6 mM CA for 3 h and then returned to medium containing varying levels of CA and assayed for EP procyclin expression. Quantitation of the EP procyclin expression at 24 h is shown.