Figure 3.

The commitment to differentiation requires new protein synthesis. (A) Schematic of the experimental regimen. (B and C) Stumpy forms were incubated with 6 mM CA in the absence (B) or presence (C) of CHX. The expression of EP procyclin was measured at 0, 1, 2, 3, or 24 h. At each time point, some cells were washed free of CHX and incubated until 24 h, at which point their expression of EP procyclin was determined. Cells maintained for 24 h in CHX were swollen and immotile; here, detection of EP procyclin probably reflects nonspecific antibody trapping by dead cells or the expression of some EP procyclin as cells lose viability in CHX. Quantitation of the EP procyclin expression at each time point is shown.