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Protein synthesis “sets the clock” for differentiation. (A) Schematic of the experimental regimen. (B and C) Stumpy forms were exposed to 6 mM CA in the absence (B) or presence (C) of CHX. After 3 h, the cells were washed free of CHX and exposed to CA and then monitored for EP procyclic expression after 1, 2, 3, and 24 h. EP procyclin expression commenced at 1 h, confirming the removal of CHX. Quantitation of the EP procyclin expression at each time point is shown.
