Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 26;211(2):455–468. doi: 10.1083/jcb.201506114

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Domingo Sananes et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — Depletion of NRK does not affect growth or differentiation of bloodstream-form trypanosomes. (A) Schematic representation of NRKA/NRKB. The two encoded proteins are 97% identical and hence indistinguishable by RNAi-based functional analysis. (B) Protein expression profile of NRKA/NRKB. Western blot of proteins from slender (Sl), stumpy (St), and procyclic (Pc) forms reveals that NRK is expressed in stumpy forms. α-Tubulin is used as a loading control. (C) RNAi against NRKA/NRKB. Two independent slender-form AnTat 1.1 lines were induced for RNAi and the expression of NRK monitored by Western blotting. The protein was depleted after 48-h induction in each case. An antibody to EF1α provided the loading control. (D) NRK RNAi does not result in a growth defect of parasites grown in vivo. When grown in vivo the induced parasites developed from slender to intermediate and stumpy forms with equivalent kinetics to parental and uninduced cell lines. Two replicate independent cell lines were analyzed in parallel, both with and without doxycycline (Dox) induction (each analyzed once, with n = 250 cells at each time point in each replicate). White boxes represent slender cells, gray boxes represent intermediate cells, and black boxes represent stumpy cells.