Figure 1.

HSV-1 capsids localize to dynamic MTs during early infection. (A and B) NHDFs were mock infected (0 h) or infected with HSV-1 at MOI 20 for the indicated times in hpi. (A) Whole cell extracts were analyzed by immunoblotting with antibodies against the total or acetylated forms of tubulin. Detection of the major capsid protein ICP5, was used to illustrate infection of samples at each point, with overexposed (OE) blots showing the presence of viral capsids in samples as early as 1 hpi (B) Fixed samples were stained for Ac-tubulin (Ac-MTs; red) or capsid protein, ICP5 (green). Nuclei were stained using Hoechst. Samples were imaged by widefield microscopy. Green and yellow arrowheads point to representative capsids with no obvious localization to Ac-MTs. Magnified regions further illustrating this are highlighted by green and yellow boxes. (C) NHDFs were infected at MOI 20 for 1 h. Fixed samples were stained for ICP5 (green), Tyr-tubulin (blue), or Ac-tubulin (red). Representative confocal planes are shown. Arrowheads point to examples of capsids localized to Tyr-MTs with little or no marks of acetylation.