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. 2015 Oct 26;211(2):261–271. doi: 10.1083/jcb.201505067

Figure 3.

Figure 3.

The membrane surrounding LDs in yft2Δ scs3Δ cells is the ER. (A) Wild-type and yft2Δ scs3Δ cells expressing ER luminal marker RFP-HDEL were grown to mid-logarithmic phase and stained with BODIPY 493/503 to visualize LDs. Optical sections showing consecutive 0.4-µm z-section images are shown. Arrowheads indicate close association of LDs with the ER. (B and C) Visualization of LDs in scs3Δ yft2Δ cells using cryo-EM. Red arrowheads indicate the ribosomes. Yellow arrows indicate the ER lumen, and green arrows denote the ER membrane. (D–F) Immunogold EM images of scs3Δ yft2Δ cells expressing ss-GFP-HDEL using anti-GFP antibodies. Arrowheads indicate the gold particles. CW, cell wall; PM, plasma membrane. (G) Model of LD growth in wild-type and scs3Δ yft2Δ cells.