Fig. 1.

Screening of antitumor activity of benzofuroxan compounds in vitro and in vivo against murine melanoma. a Cytotoxicity of 23 compounds in B16F10-Nex2 cells. Compounds were incubated with tumor cells at 100 μM for 16 h and viability was assessed by MTT method. b Antitumor activity of active benzofuroxan derivatives in vivo. Number of metastatic melanoma nodules in the lung of animals after treatment with 500 μM of compounds injected i.p. For each experiment, five animals were used per group (*p < 0.05). c N-Br and N-I melanoma treatment (300 μg/day) in NOD/SCID IL-2R-gamma null mice for seven consecutive days. No. metastatic nodules were quantified using a Stereo Microscope (Nikon) (*p < 0.05)