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. 2015 Oct 27;15:807. doi: 10.1186/s12885-015-1835-3

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© Farias et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Benzofuroxan derivative effects on melanoma cells (a) Morphological effects. Arrows point to surface membrane alterations. Original magnifications, 100× left panels; 200× right panels; 400x inserts. b DNA condensation induced by N-Br and N-I. Yellow arrows indicate positive cells for Hoechst staining. 100×, and 400× inserts. c Quantification of Hoechst 33342 relative fluorescence in treated and control cells, shown in B; **p ≤ 0.001; *p ≤ 0.05 (d) DNA fragmentation showing a ladder pattern; A: Control; B: N-Br (16 μM); C: N-I (12 μM); D: N-Br (100 μM); E: N-I (100 μM)