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. 2015 Jun 17;6(21):18406–18417. doi: 10.18632/oncotarget.4074

Figure 3. AGPAT9 inhibited breast cancer cell migration and invasion.

Figure 3

Transwell assays showed that overexpression of AGPAT9 inhibited the migration A, B. and invasion C, D. rate of MDA-MB-231 cells, and knockdown of AGPAT9 enhanced the migration (A, B) and invasion (C, D) rate of MCF7 cells. Migration E. or invasion G. was monitored for 24 h or 48 h in the xCELLigence DP system. The cell index was measured every 15 minutes. The rate of change of cell index as a function of time was calculated as a measure of migration or invasion activity. The cell index at the end (24 hrs or 48 hrs) is shown as a bar chart F, H. The migration of cells into the wound was monitored in multiple wells using a CellVoyager CV1000 confocal scanner system. The images were acquired every 0.5 hour for 28 hours (see Supplementary Movies S1 and S2) or 35 hours (see Supplementary Movies S3 and S4). The images shown represent 0 hour and 28 hours I. The distance between the two edges of the scratch in the Lenti-AGPAT9 well was obviously greater than that of the control. The images shown represent 0 hour and 35 hours J. The distance between the two edges of the scratch in the Lenti-AGPAT9-GFP well was obviously greater than that of the control. *P < 0.05; **P < 0.01.