Table 1. Sequences of the oligonucleotide pairs used by PCR to produce deletion mutants covering the CDK1 promoter.
KpnI (GGTACC) and XhoI (CTCGAG) sites were added to the primers to direct subcloning, and are italicized and underlined. Positions of the DNA fragments relative to the transcription start site are indicated.
| Position of DNA fragment | Sense 5′→3′ | Anti-sense 5′→3′ |
|---|---|---|
| −2994 to +24 | ATGGTACCGATCTCCTGACAACGTGA | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −1935 to +24 | ACGGTACCAGCGGTAACTGTATCTT | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −1578 to +24 | ACAGCCTGGTACCCTAGAGCACTTC | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −1177 to +24 | TGGATTGGTACCATGTCTAGCTCTGTTT | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −778 to +24 | TTTAATGGTACCTAAGCCAGGCGTG | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −577 to +24 | GCCGGTACCATAAACAAAATGTAAATA | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −465 to +24 | GCGGTACCTTGAGTTTTCCATTTCCT | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −382 to +24 | AAGGTACCTATACACTCCTAACCCTAAGT | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −372 to +24 | CCGGTACCTAACCCTAAGTATTAGAAGT | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −351 to +24 | TTGGTACCGAAAGTAATGGAATCTC | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −326 to +24 | ATCTCGAGGTACCCACAATATCACTTT | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −301 to +24 | AAACACAATATCACTTTTTTGGTACCTA | CTTTGAAGCCAAGCTCGAGCAGTTT |
| −276 to +24 | TTTGAACGGTACCAATGCTGGGAGAA | CTTTGAAGCCAAGCTCGAGCAGTTT |