Table 3. DIAPH1 controls tubulin-dependent vesicle trafficking.
| pendling vesicles (%) | vesicle accumulation (%) | vesicles with linear movement (%) | track length of linear moving vesicles (% of cell length) | |
|---|---|---|---|---|
| Control | 18.7 ± 9.2 | 6.3 ± 3.2 | 64.6 ± 11.3 | 29.7 ± 4 |
| D4 | 42 ± 18* | 16.4 ± 4.9* | 6.5 ± 7.7* | 12.9 ± 0.9* |
| D5 | 51.2 ± 11.3* | 12.7 ± 3.3* | 7.8 ± 6.5* | 14.2 ± 3.6* |
| Nocod. | 50.3 ± 16.7* | 14.5 ± 5.3* | 5.0 ± 3.6* | 11.6 ± 3.2* |
| Cyto. | 18.5 ± 5.5 | 6.9 ± 3.0 | 75.9 ± 6.3 | 29.6 ± 4.9 |
Control and DIAPH1 depleted cells (D4, D5) were transfected with EGFP-BDNF to visualize vesicle trafficking. In addition, EGFP-BDNF expressing control cells were treated with 100 μM Nocodazole for 3 hours and with 1 μM Cytochalasin B for 1 hour. Mean values ± SD of three independent experiments are shown.
*
p > 0.05. Nocod; Nocodazole; Cyto; cytochalasin B.