Table 1.

Mutation frequency and mutation rate in V_HDJ_H regions

Mutated genes	Mutated/total sequences	Total mutations/total bps	Mutation frequency (mutation/bp)	Mutation rate (mutations/bp/ce 11 division)a
Endogenous VH4-DXP′1-JH6
Ramosb	34/240	40/71,280	5.6 × 10−4	2.8 × 10−5
Ramosc				2.7 × 10−5
Exogenous VH1-DXP′1-JH5
P-VDJ-iEµ-Cγ1	24/192	31/70,848	4.4 × 10−4	2.2 × 10−5
P-VDJ-Cγ1	26/386	29/141,696	2.0 × 10−4	1.0 × 10−5
P-VDJ-iEµ-Cγ1-hs1,2	36/336	44/123,984	3.5 × 10−4	1.8 × 10−5
P-VDJ-Cγ1-hs1,2	47/432	50/159,408	3.1 × 10−4	1.6 × 10−5
P-VDJ-iEµ-Cγ1-hs3-hs4	26/192	27/70,848	3.8 × 10−4	1.9 × 10−5
PCMV-VDJ-iEµ-Cγ1Cγ 1	12/96	29/35,424	8.2 × 10−4	8.2 × 10−5d
PCMV-VDJ-Cγ1	11/96	16/35,424	4.4 × 10−4	4.4 × 10−5d
pEBVHis-VDJ-iEµ-Cγ1	11/96	12/35,424	3.4 × 10−4	3.4 × 10−5d

^aMutation rate calculated based on 20 cell generations with a doubling time of 36 h.

^bData from this study.

^cData from Sale and Neuberger (1998).

^dMutation rate calculated based on 10 cell generations.